LOCUS       40924_31685       13961 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pMpGWB310 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13961)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T.
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha
  JOURNAL   PLoS ONE 10 (9), E0138876 (2015)
  PUBMED    26406247
REFERENCE   2  (bases 1 to 13961)
  AUTHORS   Nishihama R, Ishizaki K, Kohchi T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, 
            Graduate School of Biostudies; Kitashirakawa-oiwake-cho, Sakyo-ku, 
            Kyoto, Kyoto 606-8502, Japan URL :http://www.lif.kyoto-u.ac.jp/e/
REFERENCE   3  (bases 1 to 13961)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13961)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2015"; volume: "10"; issue: "9"; pages: "E0138876"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, Graduate 
            School of Biostudies"; volume: " Kitashirakawa-oiwake-cho, Sakyo-ku,
            Kyoto, Kyoto 606-8502, Japan URL :http"; pages: 
            "//www.lif.kyoto-u.ac.jp/e"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13961
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      315..2043
                     /label=MpEF1alpha promoter and 5'UTR
                     /note="MpEF1alpha promoter and 5'UTR"
                     /regulatory_class="promoter"
     protein_bind    2057..2181
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2218..2248
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2302..2958
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             3303..3605
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(3649..3773)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3784..3849
                     /label=3xFLAG
                     /note="three tandem FLAG(R) epitope tags, followed by an 
                     enterokinase cleavage site"
     terminator      3868..4120
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4154..4170)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4178..4194
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4202..4232)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4247..4268)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     polyA_signal    complement(4353..4528)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4536..6572)
                     /codon_start=1
                     /gene="mALS"
                     /product="acetolactate synthase"
                     /label=mALS
                     /note="from Marchantia polymorpha; contains triple
                     amino-acid subustitutions (P207S/R208S/W582L)"
                     /protein_id="BAS49909.1"
                     /translation="MASISGCAAVPLSIASLEKVQPSARSCCKGAKELKVRSGFFSGAA
                     LRGHAYNTSRKTERSLEVKAQAVSEKIQSAVDVSGPRLSSGKSLVAPTPSDFQSRYKDD
                     EPRKGADILVEALEREGIRTVFAYPGGASMEIHQALTRSSSIRNILCRHEQGEIFAAEG
                     YAKSSGRVGVCIATSGPGATNLVTGLADALLDSVPLVAITGQVSSRMIGTDAFQETPIV
                     EVTRSITKHNYLVMNIDDIPRVIREAFFLAASGRPGPVLVDIPKDVQQQMAIPDWSQPM
                     QLKGYLQRLPRPPEPTLIEQIIRLISSSKKPVVYSGGGCNHASEELREFVELTGIPITS
                     TLMGLGAYPESDPLSLKMLGMHGTVYANYAVDQADLLLAFGVRFDDRVTGKLETFASRS
                     SIVHIDIDPAEIGKNKQPHVSMCADVKPALSEINALLKSQKRTFDFSNWVAELEEQKQK
                     WPMKFPVFEDAIAPQSAIQTLYELTGGNAIISTGVGQHQMWAAQWFKYNEPRRWLTSGG
                     LGAMGFGLPSALGAAATQPDIPVIDIDGDGSFVMNVQELATIFVEKLPVKIMILNNQHL
                     GMVVQLEDRFYKANRAHTYLGDPDHEEQIYPDFCTVADGFKVPNARVVKKDELRAAIQT
                     MLDSPGPYLLDVIVPHQEHVLPMIPGGASFKEIITEGDGRTIY"
     gene            complement(4536..6572)
                     /gene="mALS"
                     /label=mALS
     promoter        complement(6612..7288)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    complement(7483..7507)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8028..8816
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      9065..9653
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(9839..9979)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10323..10517)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10586..11656)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(12088..12714)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttggcc cgggcaaatg agtcacacac attgttgaga gacatatcaa aataactcaa
      361 ttgtaaggta agttgataga tttcccagtt aaatgcatta ttttaacaat atttgtatct
      421 caaattttca aaatcatatc aaatttgcag atcttgatga tgattcacaa ctctgtcttc
      481 gatttttgat tgttaacggg tatcgatatt tgggtaactt aaaaagaatt tgctaaagat
      541 cttaaaaaac agattaaaaa aacctttcaa aatagctaat cctttaaatc cgtttcacaa
      601 tataatgctc attgatgata atcgaaaaga ttccatttta aagtgcatat aaattgctta
      661 attatctctt tctgtctcgt aatattttgt tctctcctat tggatcggac ccgcatcttc
      721 tcgatcagtt cactatacta atatttggtc aacttcaaga gagattagaa cttcgttctc
      781 caaagtgtaa aggccgacaa gcagaaaagg aaacttcaat cagaagcctt ctaggttttc
      841 ccgcgtccca aaaagcgaag ggaggacggg gaggacggtg aggccagaag aagaaagcaa
      901 cagtaaaatc tcatgtgatt gcgtgcatgt catggatcta tgggatgcta gtccaccgtt
      961 tggaagtatg tgcagaagga gaaaggcgcg ggaggccaaa agcgacttcg gccacctctc
     1021 tactttctag aaagtttgct cgcagcttgt aataagcgcg gaaaaggtac tcgaaaaacc
     1081 ggagcaatcg gtgtggtatc agcagtcggg ttcgctaaac ctacgtcttc ttattttgga
     1141 atgttaaatt gaaagggcgt gccggcccta agcacggacg atgtgacaga cggaggagtg
     1201 tgagcaattc ggatgacgat gctggccggc agtcacgaca aagagtccga aggggcatgg
     1261 acagggcaat atataaatgg aaaagaggct ttgaatattc ctaacccttc gtcgtcggcc
     1321 tcccaacttg ccctcgcatg ctgaattggc cgcctatctg cgaatggccg gccgccgacc
     1381 ctaagtcgca ataacttgaa cggagccgat gcaaagtcct tatcgaacgc tcgttattat
     1441 aaacggggca gacctaggta tttaattata tcaaaaccct ccgcaatttc gctaatcatt
     1501 ccctaatttc ttctactttg gcgtctagtg ctgtggtcgc gcttgctctc aaagctcgct
     1561 gggaggcagt ggagcgtctg gcttaaaatc gatcgccgca accctagagt tcctttcact
     1621 tctttctctc ttgcgtggag agcatcgaca tcgaagagct gcatcttgtc ttctggtatg
     1681 cgccttgtgc cctcgtctct gtacgtcgtt acgattgctc gatattgtga ttgtctagac
     1741 ctgaaacccg actggagctt ttacgtggtg tcgattgggg cctcgagcga gtgatttttt
     1801 aggttcgttt tagccaagta catcccactt tggtcagtcc tgttatctcg gtaaggtgtt
     1861 cagttcggtc tctggtgcgg aggtgcctcg taagtttcct gcatgataga tttgtctatc
     1921 cttgacttga agaggagtcg aaggtcagcg aggttggctg tgtgctatgg acgtcggtgc
     1981 tccttggttc cgtgcttcta tgggtgttgc ctcaacagta ttgatgtgcc tgcagaaagg
     2041 ttgactagag ttatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat
     2101 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
     2161 acaacatatc cagtcactat ggcggcccct cgaggggccg cattaggcac cccaggcttt
     2221 acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc ggcgagattt
     2281 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
     2341 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
     2401 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
     2461 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc
     2521 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
     2581 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
     2641 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
     2701 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
     2761 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
     2821 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
     2881 gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
     2941 gagtggcagg gcggggcgta aagatctgga tccggcttac taaaagccag ataacagtat
     3001 gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt
     3061 atgtcaaaaa gaggtgtgct atgaagcagc gtattacagt gacagttgac agcgacagct
     3121 atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca
     3181 gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc
     3241 tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca gggactggtg
     3301 aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg
     3361 tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac
     3421 gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa
     3481 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag
     3541 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
     3601 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg
     3661 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
     3721 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggttgataac
     3781 agcgactaca aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac
     3841 gatgacaagt agagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta
     3901 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
     3961 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
     4021 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
     4081 gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattggt tccggaacca
     4141 attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     4201 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     4261 tcacattagg ctgaattagg cgcgcctatt tctgaattca acacattgcg gacgttttta
     4321 atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact ggattttggt
     4381 tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac atactaaggg
     4441 tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaaattc ccttatctgg
     4501 gaactactca cacattatta tggagaaact cgaagttagt atatagtcct tccatcaccc
     4561 tctgtgataa tctccttaaa acttgctccc ccaggaatca taggcagaac gtgctcctgg
     4621 tggggaacta tcacatccag taaataagga ccgggagaat ctagcatagt ttgaattgct
     4681 gctcttagtt catccttctt caccacacgc gcatttggca ccttgaaacc gtctgcaact
     4741 gtacagaagt cggggtagat ctgctcctcg tgatctggat cacctaagta agtatgtgct
     4801 ctgttggcct tgtagaatct atcctccaat tgtacgacca ttcctagatg ttggttgttt
     4861 aagatcatga tcttcacggg taacttctcc acaaaaattg tagccaactc ctgtacgttc
     4921 atcacaaagc taccatcgcc atcgatgtcg atcacaggaa tatctggttg agttgcggca
     4981 gcccccaaag cagaaggaag tccgaatccc atggctccaa gacctccaga tgtcaaccaa
     5041 cgccgcggtt cgttgtactt gaaccactgg gctgcccaca tttgatgttg gccaacacca
     5101 gtagatatga tggcatttcc accagtgagt tcgtacagcg tctgtatggc agattgagga
     5161 gcaatggcat cctcgaatac gggaaacttc attggccatt tctgcttttg ttcttctagt
     5221 tctgccaccc agttggagaa gtcgaaagtc ctcttttgac tcttcaagag ggcattgatt
     5281 tcacttagcg caggtttcac atcagcacac atggacacat ggggctgttt gttctttcca
     5341 atttcagcag ggtcaatatc gatatgaacg atgctggacc tgctagcaaa agtctccagt
     5401 ttaccagtca ctcgatcgtc gaaacgcact ccaaatgcca acaacagatc tgcttggtcc
     5461 acggcgtagt tggcgtaaac agttccatgc attccaagca ttttcaaact taatggatca
     5521 gactcgggat acgctcccaa acccattaaa gtcgatgtga taggaattcc ggtaagctcg
     5581 acaaattccc tcaactcttc gctggcgtga ttgcaacctc ctccagaata cactacaggc
     5641 ttcttagaac tagaaatgag tcttataatc tgctcgatta acgttggttc cggtggccga
     5701 ggcagtcgtt gtaagtagcc cttcagttgc attggctgag accaatctgg aatagccatt
     5761 tgctgttgca cgtccttggg aatatctacc aagactgggc ccggcctacc tgaagctgcc
     5821 aaaaagaaag cctccctgat tacgcgaggg atgtcatcaa tgttcataac aaggtaattg
     5881 tgcttggtga tcgatctcgt aacctccaca attggagttt cttgaaaagc atcggtacca
     5941 atcatcctac tggatacttg acctgtaatg gccactagtg gcacactgtc caagagggca
     6001 tcagcgagcc cggtaacaag attggtcgct ccaggaccgg atgtggctat acaaacccct
     6061 actcgtccag acgatttagc ataaccttca gcagcgaaaa tctccccctg ctcgtggcga
     6121 cagagaatgt tgcggatgct gctggaacgt gtaagagcct ggtgaatctc catagacgct
     6181 ccgccgggat aagcgaacac cgttctaatc ccttctcgct caagagcctc caccaaaatg
     6241 tcggctcctt tgcggggctc gtcatccttg tagcgagact gaaagtcgga cggggttggt
     6301 gcaacgagtg acttcccgct gctaagccgt ggtccactca catcgactgc agactggatc
     6361 ttctcgctaa cagcttgggc tttcacttcc agtgaacgct cagtcttccg agaggtgttg
     6421 tatgcatgtc ccctcagagc ggctcccgag aagaaaccac tcctcacctt tagctcttta
     6481 gctcccttgc aacaagacct tgccgatggc tgcaccttct ccaggctcgc tatgctcaaa
     6541 ggcacagctg cgcatccact aattgacgcc atgctcgaga gagatagatt tgtagagaga
     6601 gactggtgat ttcagcgtgt cctctccaaa tgaaatgaac ttccttatat agaggaaggg
     6661 tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa
     6721 tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt
     6781 gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta
     6841 tcgcaattat ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga
     6901 cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc
     6961 tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg
     7021 tcgtgctcca ccatgttcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc
     7081 tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc
     7141 atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt
     7201 ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc
     7261 cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt
     7321 gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttggcaagct gctctagcca
     7381 atacgcaaac cgaattcaga aataaattca gcctaattcg gcgttaattc agtacattaa
     7441 aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat
     7501 cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga
     7561 tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac
     7621 tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca
     7681 cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt
     7741 gatcaattcg ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
     7801 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     7861 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     7921 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     7981 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg
     8041 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     8101 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     8161 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     8221 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     8281 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     8341 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     8401 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     8461 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     8521 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     8581 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     8641 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     8701 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     8761 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat
     8821 gtctagctag aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt
     8881 agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta
     8941 tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa
     9001 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     9061 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     9121 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     9181 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     9241 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     9301 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     9361 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     9421 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     9481 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     9541 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     9601 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     9661 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     9721 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     9781 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     9841 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     9901 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     9961 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
    10021 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
    10081 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc
    10141 ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc
    10201 cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg
    10261 agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca
    10321 gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg
    10381 aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt
    10441 acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa
    10501 tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt
    10561 gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc
    10621 ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg
    10681 gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg
    10741 cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg
    10801 cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg
    10861 ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag
    10921 ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc
    10981 taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac
    11041 gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct
    11101 gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca
    11161 cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg
    11221 aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg
    11281 aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac
    11341 gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt
    11401 catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg
    11461 acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt
    11521 gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg
    11581 ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg
    11641 gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg
    11701 gcagacaacc cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc
    11761 cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat
    11821 tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata
    11881 gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct
    11941 ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg
    12001 ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc
    12061 tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc
    12121 ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc
    12181 ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta
    12241 cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac
    12301 gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac
    12361 caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc
    12421 ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac
    12481 gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac
    12541 aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc
    12601 ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc
    12661 gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc
    12721 cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag
    12781 ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc
    12841 aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc
    12901 cgcacggttg agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc
    12961 tcgatctctt cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt
    13021 gttcctcttg gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct
    13081 cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg
    13141 cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc
    13201 cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg
    13261 ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga
    13321 ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg
    13381 gcgtggtggt gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga
    13441 tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca
    13501 ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc
    13561 gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg
    13621 cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag
    13681 cggcggcgct cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta
    13741 tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg
    13801 taacttagga cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc
    13861 cgactgcact atagcagcgg aggggttgga tcaaagtact ttgatcccga ggggaaccct
    13921 gtggttggca tgcacataca aatggacgaa cggataaacc t
//
